人ERCC1基因启动子荧光素酶报告基因质粒的构建

      人ERCC1基因启动子荧光素酶报告基因质粒的构建

       目的：构建人切除修复交叉互补基因1（excision repair cross complementation 1，ERCC1）启动子荧光素酶报告基因质粒。方法：以人基因组DNA为模板，扩增ERCC1启动子，将其重组到荧光素酶报告基因载体pGL4 Basic中，测序验证构建的质粒中包含ERCC1启动子序列。结果：测序结果正确，质粒pGL4-ERCC1-P1构建成功。结论：人ERCC1启动子荧光素酶报告基因质粒构建成功，可以用于后续基因表达调控的研究。

      [Abstract] Objective：To construct a human excision repair cross complementation 1（ERCC1） promoter luciferase reporter plasmid.

Method：The ERCC1 promoter from human genomic DNA was amplified by PCR，and was inserted into the pGL4 Basic vector.The amplified DNA sequence was confirmed by sequencing.Result：DNA sequencing verified the successful construction of the plasmid pGL4-ERCC1-P1.Conclusion：The human ERCC1 promoter luciferase reporter gene vector is successfully constructed，which can be use for further study on gene expression regulation.

      [Key words] ERCC1； Luciferase reporter gene； Promoter